TB-500 10mg
Identity
- CAS number
- 77591-33-4
- Molecular formula
- C212H350N56O78S
- Molecular weight
- 4963.44
Physical form
- Appearance
- White lyophilised powder
- Purity
- 99%+ (HPLC)
- Solubility
- Soluble in bacteriostatic water
Handling
- Storage
- -20C, protected from light
- Shelf life
- 24 months from date of manufacture, sealed
Also known as Thymosin beta-4 fragment, TB4 fragment, Thymosin b4
£32.00
Synthetic thymosin beta-4 fragment, 10mg lyophilised. Used in actin regulation and cell migration research.
25 in stock
- Dispatch Same day if you order before the cut off, tracked
- Documentation Your batch certificate is emailed when it ships
- Storage -20C, protected from light
Worth knowing: a lot of the clinical research covers full length thymosin beta-4 rather than the TB-500 fragment. Check the methods section if you are comparing studies.
Product description
A synthetic peptide corresponding to the active region of thymosin beta-4, the actin sequestering protein found in most mammalian cell types. Supplied lyophilised in a 10mg vial.
Research context
Research using TB-500 concentrates on actin regulation and the cell migration that follows from it. Because actin dynamics underpin how cells move into a wound bed, the compound appears frequently in tissue remodelling models and in work on cardiac and corneal repair. The distinction between TB-500 and full length thymosin beta-4 matters in a write up and is worth stating explicitly in methods.
Handling notes
Reconstitute with bacteriostatic water, adding solvent slowly against the vial wall. TB-500 dissolves readily and should give a clear solution within a minute or two of gentle swirling. A cloudy solution usually means the wrong solvent rather than a bad batch. Store frozen, aliquot after reconstitution.
Worth knowing
Frequently run alongside BPC-157 in repair models, and available as a combined vial for protocols calling for both.
Documentation
This batch ships with its own certificate of analysis showing HPLC purity and mass spectrometry identity confirmation. The batch reference is recorded against your order, so a result generated months from now can be traced back to this specific lot.
Intended use
Supplied strictly for laboratory research use. Not a medicine. Not for human or veterinary consumption, and not for any diagnostic or therapeutic use.
Research background
The short version
Usually bought alongside BPC-157, and the two are studied together constantly. TB-500 is a fragment of a protein that controls how cells change shape and move, which is the first thing repair cells have to do.
TB-500 is a synthetic peptide corresponding to the active region of thymosin beta-4, an actin sequestering protein present in most mammalian cell types. The distinction between the fragment and the full protein matters when reading the literature, because a good deal of it concerns the full protein.
Where it came from
Thymosin beta-4 was characterised as a major actin binding protein in the 1980s. The fragment supplied here corresponds to the region associated with its actin binding activity.
How it is thought to work
Thymosin beta-4 binds monomeric actin and regulates the pool available for filament assembly. Because actin dynamics underlie how cells change shape and move, this is the proposed basis for observations in cell migration and tissue remodelling work.
How much of the full protein activity the fragment reproduces is not fully resolved, and studies do not always distinguish clearly between the two.
What it is studied for
What the published research found, and what it was tested in. We list the model against each one so you can judge how far the findings go.
- Actin regulation and cell migration The core mechanistic line. Effects on the monomeric actin pool and on the rate at which cells migrate in culture. What they found Sequestration of monomeric actin, reducing the pool available for filament assembly, with increased cell migration rates observed in culture. Tested in Cell culture
- Corneal and epithelial repair One of the better developed areas for thymosin beta-4, including some human clinical work on the full protein rather than the fragment. What they found Accelerated corneal epithelial healing in animal models. Some human trial data exists for full length thymosin beta-4, not for the TB-500 fragment. Tested in Animal models, some human trials of full length TB4
- Cardiac tissue models Investigated for effects on cell survival and migration following experimental injury. What they found Increased cardiomyocyte survival and migration following experimental injury in rodent models, with effects attributed to actin regulation. Tested in Rodent models
Further reading
- Thymosin beta-4: a multi-functional regenerative peptide Read the paper
Specification
| CAS number | 77591-33-4 |
|---|---|
| Molecular formula | C212H350N56O78S |
| Molecular weight | 4963.44 |
| Purity | 99%+ (HPLC) |
| Appearance | White lyophilised powder |
| Solubility | Soluble in bacteriostatic water |
| Storage | -20C, protected from light |
| Shelf life | 24 months from date of manufacture, sealed |
What you get
- The vial TB-500 10mg. Sealed and labelled with the compound and its batch reference.
- Certificate of analysis For your batch specifically. Emailed when your order ships and available on this page now.
- Cold chain packaging Gel pack and insulation. We ship these early in the week so nothing sits in a depot over the weekend.
- Plain outer packaging Plain on the outside for your privacy. Everything inside is clearly labelled.
How it compares
How this compares to the compounds people usually weigh it against. Only the specs that actually differ are shown.
| Specification | BPC-157 10mg | This product TB-500 10mg | BPC-157 and TB-500 Blend 10mg |
|---|---|---|---|
| Molecular weight | 1419.53 | 4963.44 | |
| Price | £34.00 | £32.00 | £48.00 |
Questions about this compound
How should TB-500 10mg be stored?
Store the sealed vial at -20C, protected from light. Sealed and held at that condition it carries a shelf life of 24 months from date of manufacture, sealed from the date of manufacture. Once reconstituted, shelf life shortens considerably and the solution should be aliquoted before freezing rather than thawed repeatedly.
How is TB-500 10mg reconstituted?
Soluble in bacteriostatic water. Add the solvent slowly down the inside wall of the vial rather than directly onto the material, then swirl gently to dissolve. Do not shake, vortex or sonicate to speed it up. A cloudy solution that will not clear usually means the wrong solvent rather than a poor batch.
What purity is this batch and how is it verified?
This item is supplied at 99%+ (HPLC), measured by high performance liquid chromatography. Identity is confirmed separately by mass spectrometry. Both results appear on the certificate of analysis for the batch you receive, not for a previous lot.
Can I see the certificate of analysis before ordering?
Yes. It is linked in the documentation section of this page. If no certificate is shown against this listing, ask before ordering rather than after.
Is TB-500 10mg the same as the other names it appears under?
Yes. TB-500 10mg also appears in the literature as Thymosin beta-4 fragment, TB4 fragment, Thymosin b4. They refer to the same compound, which is worth knowing when searching for prior work because results are split across the names.
Can this be used in humans or animals?
No. This is supplied strictly as a laboratory reagent for in vitro research. It is not a medicine, it is not licensed for human or veterinary use, and it should not be administered to a person or an animal. We do not provide dosing or administration guidance.
Handy guides
- Reconstitution and solvents Choosing a solvent, adding it without damaging the peptide, working out concentrations, and the handling errors that cost people a vial.
- Storage and handling Temperature, light, reconstitution and shelf life, and what actually degrades a compound in practice.
- Freeze thaw cycles and shelf life What degrades a peptide in practice, how much a freeze thaw cycle actually costs you, and how to plan aliquots so it stops mattering.
- How to read a certificate of analysis A section by section walkthrough of a peptide certificate: what each figure means, what it does not mean, and the checks worth making before you accept a batch.
